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Anti-Roquin-1/2, clone 3F12 Antibody
产品别名
Anti-Roquin-1/2, clone 3F12 Antibody
Roquin-1, Roquin, RING finger and C3H zinc finger protein 1, RING finger and CCCH-type zinc finger domain-containing protein 1, RING finger protein 198
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Roquin-1 shares 46% identity with Roquin-2 and both serve the same function and belong to the Zinc finger family of RNA binding proteins. Roquin-1/2 are cytoplasmic RNA suppression proteins that specifically bind selective RNAs and promote the deadenylation of mature mRNAs and their degradation. Roquin-1/2s targets include HMGXB3, ICOS, IER3, NFKBID, NFKBIZ, PPP1R10, TNF and in many more critical RNAs. In particular, because of their impact on ICSO and gamma interferon and T helper cell activation, Roquin-1/2 are being examined as mediators of the systemic inflammation response and controllers of adaptive and innate immune responses in autoimmune disease. Roquin 1/2 are widely expressed and localized in the cytoplasm and/or P bodies and cytosolic stress granules when under cellular stress. |
| Specificity【特异性】 | This antibody specifically detects both Roquin-1 & Roquin-2 (Vogel, K.U., et al. (2013). Immunity. 38:655-668). |
| Immunogen【免疫原】 | Recombinant protein corresponding to human Roquin-1/2. |
| Application【应用】 | This Anti-Roquin-1/2, clone 3F12 Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of Roquin-1/2. Western Blotting Analysis: A representative lot detected Roquin-1/2 in both Roquin-1 & Roquin-2 in normal MEFs, only Roquin-2 and Roquin-1 deficient MEFs, and neither protein in Roquin-1/2 deficient MEFs. This antibody also detected Roquin-1 and Roquin-2 in Mouse Spleen, Lymph Node, Thymus, Brain & Lung tissue, CD4+ T cells and Thymocyte lysates (Vogel, K.U., et al. (2013). Immunity. 38:655-668). Immunoprecipitation Analysis: A representative lot immunoprecipitated Roquin-1/2 in MEF cell lysate (Vogel, K.U., et al. (2013). Immunity. 38:655-668). |
| Physical form【外形】 | Protein G Purified Format: Purified |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | rat |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | 3F12, monoclonal |
| species reactivity | human, mouse |
| technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
| isotype【同位素/亚型】 | IgG2aκ |
| NCBI accession no.【NCBI登记号】 | NP_742068 |
| UniProt accession no.【UniProt登记号】 | Q5TC82 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... RC3H1(149041) |
产品说明
| Target description【目标描述】 | 125 kDa calculated |
| 【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
