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Anti-SCRAPPER Antibody
| 产品编号： | 4114064 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-SCRAPPER Antibody
F-box, Leucine rich repeat protein 20, FBXL20, FBl2, FBL20
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Members of the F-box protein family, such as SCRAPPER, are characterized by an approximately 40-amino acid F-box motif. SCRAPPER is selectively expressed in the brain, broadly expressed within the mouse CNS, and is abundant at the presynaptic membrane. It has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. SCRAPPER is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check. |
| Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to human SCRAPPER. |
| Application【应用】 | This SCRAPPER antibody is validated for use in WB & IHC for the detection of the SCRAPPER protein. Western Blot Analysis: 1 µg/mL from a representative lot detected SCRAPPER in 10 µg/mL of RAW264.7 cell lysate. Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected SCRAPPER in human midbrain tissue. Research Category Neuroscience Research Sub Category Developmental Neuroscience |
| Quality【质量】 | Evaluated by Western Blot in human spleen tissue lysate. Western Blot Analysis: 1 µg/mL of this antibody detected SCRAPPER in 10 µg/mL of human spleen tissue lysate. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide. Antigen Affinity Purified |
| Analysis Note【分析说明】 | Control Human spleen tissue lysate |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable western blot: suitable |
| UniProt accession no.【UniProt登记号】 | Q96IG2 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... FBXL20(84961) |
产品说明
| Target description【目标描述】 | ~48 kDa observed. Uncharacterized bands may appear at ~52 kDa, ~58 kDa and ~70 kDa in some lysates. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 2 |
