Anti-Semaphorin 4A Antibody, clone 8B5.1

32160702

Semaphorin-4A (UniProt Q9H3S1; also known as Sema B, Semaphorin-B) is encoded by the SEMA4A (also known as CORD10, RP35, SEMAB, SEMB) gene (ORP UNQ783/PRO1317; Gene ID 64218) in human. Semaphorin-4A is a membrane-type class IV semaphorin originally characterized for its role in axon guidance during neural development, Semaphorin-4A also plays a role in Th cells activation and the differentiation of Th1 and Th17 cells. In addition Semaphorin-4A is involved in angiogenesis and endothelial cells migration by modulating VEGF signaling. Consistent with its important role in Th cells, Semaphorin-4A level is reported to be elevated in the serum samples from patients with multiple sclerosis (MS) respond poorly to interferon-beta (IFN-β) therapy. Semaphorin-4A neutralizing antibodies are reported to inhibit antigen-specific T cell generation and ameliorate experimental autoimmune encephalomyelitis (EAE), an animal model of MS.

Expected to react with both spliced isoforms.

GST-tagged recombinant protein corresponding to the Sema domain of human Semaphorin 4A.
Epitope: Sema domain

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected Semaphorin 4A in 10 µg of human retinal pigment epithelia (RPE) ARPE-19.
This Anti-Semaphorin 4A Antibody, clone 8B5.1 is validated for use in Western Blotting for the detection of Semaphorin 4A.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling

Evaluated by Western Blotting in human monocytic leukemia THP-1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Semaphorin 4A in 10 µg of human monocytic leukemia THP-1 cell lysate.

Purified by Ion-Exchange Chromatography
Purified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.
Format: Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified antibody

primary antibodies

8B5.1, monoclonal

human

western blot: suitable

IgMκ

NP_001180229

Q9H3S1

wet ice

~85 kDa observed. 83.6 kDa (isoform 1) and 69.1 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

Stable for 1 year at 2-8°C from date of receipt.

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10 - Combustible liquids

WGK 2