Anti-Set1A Antibody

32160702

NA.41

Set1A is a histone methyltransferase (HMT) that is part of the SET1 HMT complex. This protein specifically methylates lysine 4 of histone H3 if the adjacent lysine at position 9 is also unmethylated. Studies demonstrate that Set1A and Set1B localize to largely non-overlapping regions of the chromosome. This suggests Set1A and Set1B bind to unique sets of target genes and that each protien makes non-redundant contributions to the epigenetic control of chromatin structure and gene expression.

GST-tagged recombinant protein corresponding to human Set1A.

Use Anti-Set1A Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Set1A also known as SET domain containing 1A, Set1/Ash2 histone methyltransferase complex subunit SET1.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histone Modifying Proteins
Chromatin Immunoprecipitation (ChIP):
A representative lot of this antibody was reported to work in ChIP. See Lee, J.H., et al.(2008). Mol Cell Biol. 28(2):609–618.

Immunocytochemistry:
A representative lot of this antibody was reported to work in immunocytochemistry. See . Lee, J.H., et al. (2007). J Biol Chem. 282(18):13419-13428.

Evaluated by Western Blot in PC3 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected Set1A on 10 µg of PC3 cell lysate.

Unpurified
Rabbit polyclonal serum containing 0.05% sodium azide.

Control
PC3 cell lysate

rabbit

100

serum

primary antibodies

polyclonal

mouse, rat, human

ChIP: suitable
western blot: suitable

NP_055527

O15047

wet ice

~186 kDa observed. A cross-reacting band appears at ~45 kDa

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1