Anti-SIP1 Antibody, clone 6E5

32160702

NA.41

Smad-interacting protein 1 (SIP1, SMADIP1), also known as Zinc finger E-box-binding homeobox 2 (ZEB2), and Zinc finger homeobox protein 1b (ZFHX1B, ZFX1B), is a transcriptional inhibitor that binds to DNA sequence 5′-CACCT-3′ in different promoters and interacts with activated SMADs. ZEB2 has been shown to repress the transcription of the E-cadherin gene and induce epithelial-mesenchymal transition, a genetic program controlling cell migration during embryonic development and wound healing in vitro. It is suggested that ZEB2 contributes to tumor progression since it’s been demonstrated to protect cells from DNA damage-induced apoptosis. Recent studies have shown that ZEB2 is often observed in the cytoplasm in numerous cancer tissues, implying that its localization may be regulated in normal and tumor tissues. Mutations in this gene are also associated with Mowat-Wilson syndrome (MWIS), also known as Hirschsprung disease-mental syndrome.

His-tagged recombinant protein corresponding to the N-terminus of Human SIP1.
Epitope: N-terminus

This antibody has been shown to work in Immunocytochemistry and Immunohistochemistry applications (Oztas et al., 2010).
Anti-SIP1 Antibody, clone 6E5 is an antibody against SIP1 for use in western blotting, ICC & IHC.
Research Sub Category
Pluripotent & Early Differentiation
Research Category
Stem Cell Research

Evaluated by Western Blot in a SK-Hep1 cell lysate. 2ug/ml (1:500 dilution) of this antibody detected SIP-1 in a SK-Hep1 cell lysate.

Purified mouse monoclonal IgG2aκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

100

mouse

purified immunoglobulin

primary antibodies

6E5, monoclonal

human

1 mg/mL

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

IgG2aκ

NP_001165124

O60315

wet ice

human ... ZEB2(9839)

~220 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1