Anti-Somatoliberin/GHRH Antibody, clone 1B4.2

32160702

The human GHRH (also known as GHRF) gene (Gene ID 2691) encodes the 108 aa Somatoliberin preproprotein (UniProt P01286; also known as GHRH, GRF, Growth hormone-releasing factor, Growth hormone-releasing hormone, INN: Sermorelin, Sermorelin, Somatocrinin, Somatorelin). Post-translatioinal removal of the signal peptide (aa 1-20) and the two propeptide sequences (aa 21-31 and aa 76-108) releases the 44 aa mature Somatoliberin (aa 32-75) that functions as a ligand to stimulate growth hormone (GH) release from the pituitary. Variant GHRH receptors are found in several types of tumors. Antagonists against such receptors are reported to inhibit tumors growth. Defects in GHRH gene cause dwarfism and hypersecretion of somatoliberin is linked to gigantism.

Clone 1B4.2 is expected to detect prepro-, pro-, and mature somatoliberin.

Epitope: Mature somatoliberin
KLH-conjugated linear peptide corresponding to mature human somatoliberin sequence.

This Anti-Somatoliberin Antibody, clone 1B4.2 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of GHRH .
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Somatoliberin/GHRH in human cerebral cortex tissue.
Research Sub Category
Apoptosis - Additional
Research Category
Apoptosis & Cancer

Evaluated by Western Blotting in human lung tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Somatoliberin/GHRH in 10 µg of human lung tissue lysate.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified immunoglobulin

primary antibodies

1B4.2, monoclonal

human

immunohistochemistry: suitable (paraffin)
western blot: suitable

IgG1κ

NP_001171660

P01286

dry ice

~12 kDa observed. Preprosomatoliberin (12.4 kDa calculated); Prosomatoliberin (10.9 kDa calculated); Mature somatoliberin (10.4 kDa calculated). Uncharacterized band(s) may appear in some lysates.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1