Anti-Sortilin Antibody, clone 4E3.1

32160702

NA.75

Sortilin (UniProt Q99523; also known as 100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3) is encoded by the SORT1 (also known as LDLCQ6, NT3) gene (Gene ID 6272) in human. Sortlin belongs to the a family of sorting receptors characterized by the Vps10p domain that creates a tunnel cavity with its ten-bladed β-propeller for the binding of soluble ligands. The family members also contain a short cytoplasmic tail that harbors recognition motifs for subcellular sorting adaptor proteins. Sortilin is reproted to bind PCSK9 in the trans-Golgi network with high-affinity in HepG2 cells. Sort1 knockout mice exhibit reduced PCSK9 plasma concentration and altered PCSK9 subcellular localization in hepatocytes. Sortilin is also found to colocalize with APP in neurites and facilitates APP processing toward the production of sAPPα, possibly by optimizing APP as a substrate for α-secretase cleavage. Sortilin is initially produced with a signal peptide (a.a. 1-33) and a propeptide (a.a. 34-77) sequence, the removal of which yields the mature receptor (a.a. 78-831) with a large luminal/extracellular (a.a. 78-555) portion, a transmembrane region (a.a. 756-778), and a short cytoplasmic tail (a.a.779-831). Similar to the other two family members (MPRs and SorLA), sortilin resides both on the membrane of intracellular vesicles and on the plasma membrane. Altered sortilin expressions due to SORT1 genetic variations affect serum levels of low density lipoprotein cholesterol (LDL-C) and contribute to the LDL-C level quantitative trait locus 6 (LDLCQ6) and susceptibility to myocardial infarction.

Expected to react with both spliced isoforms reported by UniProt (Q99523).

Epitope: C-terminal region.
GST-tagged recombinant human Sortilin C-terminal fragment.

Research Sub Category
Vesicular Trafficking
Immunohistochemistry Analysis: An 1:250 dilution of this antibody from a representative lot detected Sortilin in human cerebral cortex, colon, and kidney tissue sections.
Research Category
Stem Cell Research
Anti-Sortilin Antibody, clone 4E3.1 is an antibody against Sortilin for use in Western Blotting, Immunohistochemistry (Paraffin).

Evaluated by Western Blotting in E13-14 embryonic mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Sortilin in 10 µg of E13-14 embryonic mouse brain tissue lysate.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified immunoglobulin

primary antibodies

4E3.1, monoclonal

mouse, human

immunohistochemistry: suitable (paraffin)
western blot: suitable

IgG1κ

NP_002950

Q99523

wet ice

human ... SORT1(6272)

~92 kDa observed. Target band size appears larger than the calculated molecular weights of 83.86 kDa (isoform 1) and 69.17 kDa (isoform 2) due to glycosylation. Uncharacterized band(s) may appear in some lysates.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1