Anti-STK39 Antibody, clone 5H7.1

32160702

NA.41

Serine/threonine-protein kinase 39 (STK39), also known as STE20/SPS-1 related proline alanine-rich kinase (SPAK), is a member of the SPS1 subfamily of STE20 kinases. STK39 is also very highly conserved between species and has been shown to activate the p38 pathways. STK39 also has a caspase cleavage site and these two functions may mean that STK39 plays a role in stress-activated signaling. STK39 expression is up-regulated by androgens in LNCaP human prostate cancer cells and regulated and phosphorylated by WNK1, which controls the stress signaling pathway. It is activated by WNK4 and then has the ability to stimulate the Na-K-2Cl cotransporter, the inactive version does not possess this ability. Recent research has also made a connection between STK39 and regulation of blood pressure.

This antibody recognizes the C-terminus of STK39.

GST-tagged recombinant protein corresponding to the C-terminus of human STK39.
Epitope: C-terminus

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected STK39 in 10 µg of mouse and rat brain tissue lysate.
Immunohistochemistry Analysis: A 1:500-2,000 dilution from a representative lot detected STK39 in human cerebellum sections and rat hindbrain sections tissue.
Research Category
Signaling
Anti-STK39 Antibody, clone 5H7.1 is a highly specific mouse monoclonal antibody, that targets STK39 & has been tested in western blotting & IHC.
Research Sub Category
Developmental Signaling

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected STK39 in 10 µg of human brain tissue lysate.

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified antibody

primary antibodies

5H7.1, monoclonal

mouse, rat, human

immunohistochemistry: suitable
western blot: suitable

IgG1κ

NP_037365

Q9UEW8

wet ice

human ... STK39(27347)

~60 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1