Anti-SUN1 Antibody

32160702

NA.41

SUN domain-containing protein 1 (SUN1), also known as Protein unc-84 homolog A or Sad1/unc-84 protein like 1 (UNC84A), is a component of the SUN-protein-containing complexes also called LINC (linker of nucleoskeleton and cytoskeleton) complexes. These complexes link the nucleoskeleton and cytoskeleton structures together in a scaffold like fashion. SUN1 is required for interkinetic nuclear migration and nucleokinesis as well as nucleus-centrosome coupling during radial neuronal migration and glial migration in the cortex. SUN1 also is important during meiosis and is required for proper gametogenesis as well. Additionally, SUN1 it is important in the distribution of nuclear pore complexes on the nuclear membrane. Interestingly, LINC complexes are also critical for vital functions such as hearing. For instance, recent research demonstrates that the position of the nucleus in sensory epithelial cells, partly governed by SUN1, appears critical for normal hearing.

Epitope: Perinuclear space domain
MBP-tagged recombinant protein corresponding to the perinuclear space domain of human SUN1.

Research Category
Cell Structure
Detect SUN1 using this Anti-SUN1 antibody validated for use in western blotting & ICC.
Western Blotting Analysis: A 1:1;000 dilution from a representative lot detected SUN1 in 10 µg of HepG2, Jurkat, and PC3 cell lysate.
Western Blotting Analysis: A representative lot detected SUN1 recombinant protein (Haque, F., et al. (2010). Journal of Biol. Chem. 285(5): 3487-3498).
Immunocytochemistry Analysis: A representative lot detected SUN1 in human foreskin fibroblasts cells (Haque, F., et al. (2010). Journal of Biol. Chem. 285(5): 3487-3498).
Research Sub Category
Adhesion (CAMs)

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1;000 dilution of this antibody detected SUN1 in 10 µg of A431 cell lysate.

Affinity purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human, rat

immunocytochemistry: suitable
western blot: suitable

NP_001124437

O94901

wet ice

human ... SUN1(23353)

~ 110 kDa observed. The calculated molecular weight is 90 kDa, however Sun1 has been shown as a ~80-110 kDa band in western blots (Gob, E., et al. (2011). Commun. Integr Biol. 4(4):440-442). Uncharacterized band(s) may appear in some lysates.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2