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Anti-TIF1-alpha Antibody
| 产品编号： | 4115226 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-TIF1-alpha Antibody
Transcription intermediary factor 1-alpha, E3 ubiquitin-protein ligase TRIM24, RING finger protein 82, Tripartite motif-containing protein 24
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Transcription intermediary factor 1-alpha (TIF1-alpha) is also called E3 ubiquitin-protein ligase TRIM24, RING finger protein 82, and Tripartite motif-containing protein 24. TIF1-alpha is a transcriptional coactivator that interacts with chromatin depending on histone H3 modifications, with the highest affinity for histone H3 that is both unmodified at ′Lys-4′ (H3K4me0) and acetylated at ′Lys-23′ (H3K23ac). TIF1-alpha has E3 protein-ubiquitin ligase activity, helps regulate cell proliferation and apoptosis and up-regulates transcription activation. Aberrant TIF1-alpha can cause thyroid papillary carcinoma (TPC) and is up-regulated in some breast cancers. |
| Specificity【特异性】 | Other homologies: Rat (93% sequence homology). This antibody recognizes the C-terminus of the Nuclear Receptor Binding Site (NRBS) of TIF1-alpha. |
| Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to the C-terminus of the Nuclear Receptor Binding Site (NRBS) of human TIF1-alpha. Epitope: C-terminus of the Nuclear Receptor Binding Site (NRBS) |
| Application【应用】 | Immunocytochemistry Analysis: A 1:50 dilution from a represenative lot detected TIF1-alpha in HeLa cells. Detect TIF1α using this rabbit polyclonal antibody, Anti-TIF1-alpha Antibody validated for use in western blotting & ICC. Research Category Epigenetics & Nuclear Function Research Sub Category Chromatin Biology |
| Quality【质量】 | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.1 µg/mL of this antibody detected TIF1-alpha in 10 µg of HeLa cell lysate. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Analysis Note【分析说明】 | Control HeLa cell lysate |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | rhesus macaque (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_056989 |
| UniProt accession no.【UniProt登记号】 | O15164 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... TRIM24(8805) |
产品说明
| Target description【目标描述】 | ~140 kDa observed. Uniprot descrives 2 isoforms at ~117 kDa and ~113 kDa due to alternative splicing. This protein has been observed at ~140 kDa (Gunawardena, H., et al. (2008). Rheumatology (Oxford). 47(3):324-328.). |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
