Anti-TRIM33 Antibody, clone 3B5.1

32160702

NA.41

TRIM33, also known as E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, RET-fused gene 7 protein, Rfg7, Transcription intermediary factor 1-gamma, TIF1-gamma, or Tripartite motif-containing protein 33, and encoded by the gene TRIM33/KIAA1113/RFG7/TIF1G, is an E3 ubiquitin protein ligase. Among its targets is SMAD4 for which ubiquitination facilitates its nuclear exclusion and degradation. SMAD3 is also a target for ubiquitination, and together, TRIM33′s association promotes erythroid differentiation of hematopoietic stem cells. Finally mono-ubiquitination of various targets by TRIM33 affects their ability to form complexes which often hampers their activity and thus regulates their subsequent actions. Such is the case of SMAD4 and its interaction with TGF-beta/BMP signaling. Monoubiquitination of SMAD4 by TRIM33 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. TRIM33 is localized in nucleus in nuclear dots or bodies and is expressed in most tissues and stem cells during development as well as in the adult in multiple tissues.

GST-tagged recombinant protein corresponding to human TRIM33.

Research Sub Category
Nuclear Receptors
This Anti-TRIM33 Antibody, clone 3B5.1 is validated for use in Western Blotting and Immunocytochemistry for the detection of TRIM33.
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected TRIM33 in H9 stem cells.
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Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected TRIM33 in 10 µg of K562 cell lysate.

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

ALEXA FLUOR is a registered trademark of Life Technologies

100

mouse

purified antibody

primary antibodies

3B5.1, monoclonal

human

1 mg/mL

immunocytochemistry: suitable
western blot: suitable

IgG1κ

NP_056990

Q9UPN9

wet ice

human ... TRIM33(51592)

~123 kDa observed. Uncharacterized band(s) may appear in some lysates.

Stable for 1 year at 2-8°C from date of receipt.

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12 - Non Combustible Liquids

WGK 1