Anti-Tristetraprolin Antibody

32160702

NA.41

Tristetraprolin (TTP, Nup 475, TIS11, G0S24, or ZFP36) is a member of the CCCH tandem zinc finger protein family. It is a nuclear RNA-binding protein that functions as a trans-acting factor. It is involved in post-transcriptional regulation of cytokine genes, most notably Tumor Necrosis Factor alpha (TNF-α). Upon activation by lipopolysaccharides, TTP binds TNF-α mRNA at AU-rich domains in the 3′-untranslated Region (3′-UTR) and induces deadenylation, thereby destabilizing the mRNA and tagging it for degradation. To produce this effect, TTP recruits other trans-acting factors to its C-terminal domain and mRNA-decay enzymes to its N-terminal domain. TTP is deactivated by phosphorylation driven by mitogen-activated protein kinase-activated protein kinase 2 (MK2) and p53. Therefore, TTP/MK2 regulates the biosynthesis of TNF-α and mediates inflammatory responses involving TNF-α. Knockout of TTP in mice produces arthritis, alopecia, dermatitis, among a range of other phenotypes related to overexpression of TNF-α.

KLH-conjugated linear peptide corresponding to human Tristetraprolin.

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to detect Tristetraprolin in human leukocytes. (Brooks, S., et al. (2002). Arthritis and Rheumatism. 46(5):1362–1370.)
Research Sub Category
Growth Factors & Receptors
Research Category
Epigenetics & Nuclear Function
Anti-Tristetraprolin Antibody is an antibody against Tristetraprolin for use in Western Blotting, ICC, Immunoprecipitation, RNA Binding Protein Immunoprecipitation (RIP).

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Tristetraprolin on 10 µg of A431 cell lysate.

Purified rabbit polyclonal in buffer containing PBS without 0.05% sodium azide.
Affinity purified

Control
A431 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human, mouse

RIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NP_003398

P26651

wet ice

~40 kDa observed. Uniprot gives a calculated molecular weight of 34 kDa An uncharacterized band appears at ~53 kDa in some lysates.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2