Anti-VCAM-1 Antibody, clone M/K

32160702

NA.41

Recognizes Murine vascular adhesion molecule (VCAM-1), single chain glycoprotein of Mr 100- kDa (under reducing conditions). VCAM-1 is present on the +/+ 2.4 stromal cell clone, large cells in bone marrow, and a subset of thymic macrophages. The stromal cell antigen is also detectable on the EOMA and BMS2 endothelial cell lines. The antibody reacts with all known isoforms of murine VCAM-1.

Anti-VCAM-1 Antibody, clone M/K is an antibody against VCAM-1 for use in FC, IH & IP.
Studies of cell-cell interaction

Cell adhesion assays

Inhibition of lymphopoieisis in Whitlock-Witte long-term marrow cultures

Immunoprecipitation of the VCAM-1 integrin complex

Detection of VCAM-1 cells by immunofluorescence and immunohistochemical staining: acetone or fresh frozen sections work best; although zinc formalin or PLP fixations are reported to work but they have not be verified by Chemicon.

Optimal working dilutions must be determined by the end user.

Replaces: MAB2242

Protein A purified
Format: Purified

Control
EOMA and BMS2 endothelial cell lysates

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

rat

purified antibody

primary antibodies

M/K - 2, monoclonal

mouse

Chemicon^®

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

IgG1κ

NM_001078.2
NM_080682.1

P19320

wet ice

human ... L1CAM(3897), VCAM1(7412)

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2