Anti-VEGF Antibody, Alexa Fluor^® 647 Conjugate

32160702

NA.45

VEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively). All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines. VEGF induces angiogenesis as well as permeabilization of blood vessels, and also plays a central role in the regulation of vasculogenesis. VEGF expression correlates to the degree of tumor vascularization and with increased metastatic risk. Additionally, VEGF induces endothelial cell proliferation, promotes cell migration, and inhibits apoptosis. Consequently, inhibition of VEGF signaling abrogates the development of a wide variety of tumors.

Other homologies: Other homologies: Bovine (85% sequence homology). Rat (70% sequence homology). Mouse (65% sequence homology).
This antibody recognizes VEGF at the N-terminus.

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to human VEGF at the N-terminus.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected VEGF in A431 and NIH/3T3 cells.
The unconjugated antibody (Cat. No. ABS82) is shown to be suitable also for Western blotting and immunohistochemistry (Paraffin) applications.
Research Sub Category
Growth Factors & Receptors
Research Category
Signaling

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected VEGF in HeLa cells.

Purified rabbit antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.
Affinity purified

Concentration: Please refer to lot specific datasheet.

ALEXA FLUOR is a registered trademark of Life Technologies

rabbit

100

ALEXA FLUOR^® 647

affinity isolated antibody

primary antibodies

polyclonal

human, mouse, rat

nonhuman primates (based on 100% sequence homology)

immunocytochemistry: suitable

IgG

NP_001020537

P15692

wet ice

human ... VEGFA(7422)

~55 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2