GAL4 [(1-147)-AH] from Saccharomyces cerevisiae

NA.26

Transcriptional activity is greatly stimulated by promoter-specific activator proteins. These are modular proteins, consisting of a DNA-binding domain and a regulatory (activator) domain. The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5′-CGGN5(T/A)N5CCG-3′ (5 ). A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. Linking of an acidic synthetic peptide, forming an α-helix (AH), to this GAL4 DNA-binding domain, results in a protein with an amphipathic structure. This fusion protein is able to activate transcription of a gene, bearing the GAL4 binding sites in an in vitro transcription system by targeting TFIIB in the pre-initiation complex.

Clear and colorless frozen liquid solution

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

expressed in E. coli

≥80% (SDS-PAGE)

frozen liquid

~18.6 kDa

pkg of 10 μg

avoid repeated freeze/thaw cycles

300 μg/mL

electrophoretic mobility shift assay: suitable

colorless to clear

NM_001184062

P04386

dry ice

−70℃

Saccharomyces cerevisiae ... GAL4(855828)

10 - Combustible liquids

WGK 1