Glial Fibrillary Acidic Protein, porcine

32160405

NA.41

Glial fibrillary acidic protein (UniProt F1RR02) is encoded by the GFAP gene (Gene ID 396562) in porcine. GFAP is a type III intermediate filament (IF) thought to help maintain astrocyte mechanical strength and is closely related to vimentin, desmin, and peripherin, which are involved in the structure and function of cellular cytoskeleton. GFAP is expressed in astrocytes in the central nervous system (CNS), where it is involved in many important CNS processes, including cell communication and the blood brain barrier (BBC) function. GFAP contains the characteristic tripartite domain structure among IF members, a central α-helical rod domain (a.a. 73-377) flanked by a non-α-helical N-terminal head (a.a. 1-72) and a C-terminal tail (a.a. 378-432) domain. The head and tail domains are most structurally variable among IF proteins and their contributions to filament assembly vary depending on the IF proteins. For GFAP, the tail domain is reported to be important for filament assembly. The tail domain R416W mutation is linked to the neurological disorder Alexander disease (AxD).
Purified porcine Glial Fibrillary Acidic Protein (GFAP) purified from spinal cord.

Liquid. Buffer = PBS.

a 50 kDa single band by SDS-PAGE.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Porcine

100

>99% (pure GFAP)

calculated mol wt 49.44 kDa (428 a.a. spliced isoform; NP_001231326)
calculated mol wt 53.63 kDa (468 a.a. spliced isoform; XP_005668766)

Chemicon^®

1 mg/mL

ELISA: suitable
radioimmunoassay: suitable
western blot: suitable

NM_001244397

F1RR02

dry ice

pig ... GFAP(396562)

Maintain at -20°C to -70°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2