Glucokinase from human pancreas

NA.32

Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase (Accession# NP_000153)) has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.

One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30℃. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 μg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30℃ for 30 min.

50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.

human pancreas

expressed in E. coli

≥80% (SDS-PAGE)

liquid

53.1 kDa

pkg of 10 μg

1 mg/mL

Endotoxin, tested

P35557

P35557

dry ice

−70℃

human ... GCK(2645)

12 - Non Combustible Liquids

WGK 2