Glucose-6-phosphate Isomerase human

5.3.1.9 ( BRENDA | IUBMB )

NA.54

Glucose-6-phosphate isomerase (GPI) is a homodimeric enzyme ubiquitously present in most organisms. GPI catalyzes the interconversion between glucose-6-phosphate and fructose-6-hosphate, the second step of the glycolytic pathway. In mammals, Glucose-6-phosphate isomerase also acts as an autocrine motility factor (AMF), a neuroleukin, and a maturation factor. GPI deficiency is the second most common erythroenzymopathy of glycolytic enzymes after pyruvate kinase deficiency. Inherited deficiency of the enzymatic activity of GPI causes hereditary nonspherocytic hemolytic anemia (HNSHA) in humans, a severe deficiency that can be associated with hydrops fetalis (a-thalassaemia), immediate neonatal death, and neurological impairment.

C-terminal histidine-tagged

One unit will convert 1.0 mmole of D-fructose 6-phosphate to D-glucose 6-phosphate per inute at pH 7.4 at 25 ℃.

Supplied as a solution in 50 mM Tris-HCl pH 7.5, and 50% glycerol

200

expressed in E. coli

≥95% (SDS-PAGE)

≥200 units/mg protein

−20℃

12 - Non Combustible Liquids

WGK 1