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Glutathione GSH/GSSG Assay Kit
产品性质
| Quality Level【质量水平】 | 100 |
| application(s) | pharmaceutical |
| Detection Method | colorimetric |
| relevant disease(s)【相关疾病】 | Alzheimer′s disease; Parkinson′s disease; neurological disorders; cancer; aging/geriatric diseases |
| storage temp.【储存温度】 | −20℃ |
| suitability【适用性】 | The kit is suitable for the quantitative determination of reduced and oxidized glutathione (GSH/GSSG) in whole blood, plasma, serum, urine, tissue and cell extracts. |
基本信息
| General description【一般描述】 | Glutathione, a tripeptide of glycine, glutamic acid, and cysteine, is one of the key antioxidants involved in protecting cells from damage by reactive oxygen species. Glutathione (GSH) reduces disulfide bonds in cytoplasmic proteins to cysteines, in which it is converted to its oxidized form GSSG. |
| Features and Benefits【特点和优势】 |
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| Principle【原理】 | The Glutathione GSH/GSSG Assay Kit is designed to accurately measure total, reduced and oxidized glutathione in biological samples using an enzymatic method that utilizes Ellman′s Reagent (DTNB) and glutathione reductase (GR). DTNB reacts with reduced glutathione to form a yellow product. The rate of change in the optical density, measured at A412 nm, is directly proportional to the glutathione concentration in the sample. This kit can also be used to measure oxidized (GSSG) by using a specific protocol which first scavenges all GSH with 1-methyl-2-vinylpyridinium triflate. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 3 |
| Flash Point(F)【闪点(F)】 | 188.6 °F |
| Flash Point(C)【闪点(C)】 | 87 ℃ |
