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Glutathione S-transferase Assay Kit

产品编号:4118983
规格:Sufficient for 100 colorimetric tests
包装规格:1 kit
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
41189831 kit3980
产品性质
Quality Level【质量水平】
100
Detection Method
colorimetric
relevant disease(s)【相关疾病】
neurological disorders, asthma; pulmonary disorders, diabetes; endocrinological disorders; cancer
storage temp.【储存温度】
−20℃
suitability【适用性】
The kit is suitable for Glutathione S-transferase activity determination in cell lysates, tissues, etc.
基本信息
General description【一般描述】
Glutathione transferase (GST) is a multifunctional enzyme that plays an important role in cellular detoxification. GST protects cells against foreign compounds such as carcinogens and drugs by catalyzing the attachment of glutathione to the compounds electrophilic and/or hydrophobic sites.
Features and Benefits【特点和优势】
  • Fast and sensitive. Linear detection range (20 μL sample): 2 to 80 U/L for 10 min reaction at 25 ℃.
  • Compatible with high-throughput handling systems.
Principle【原理】
The Glutathione S-transferase Assay Kit is based on the GST enzyme reaction between GSH and the GST substrate, CDNB (1- chloro-2,4-dinitrobenzene). The GST catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected spectrophotometrically at A340 nm. The rate of increase in absorbance at A340 nm is directly proportional to the GST activity in the sample.
安全信息
Pictograms【象形图】
GHS07
Signal word【警示用语:】
Warning
Hazard Statements
H317 - H319 - H412
Precautionary Statements
P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
Hazard Classifications【危险分类】
Aquatic Chronic 3 - Eye Irrit. 2 - Skin Sens. 1
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)【闪点(F)】
188.6 °F
Flash Point(C)【闪点(C)】
87 ℃
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