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HDAC-3/NCOR2 human
产品编号: | 4119138 |
规格: | recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE) |
包装规格: | 50 μG |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
HDAC-3/NCOR2 human
RPD3
Histone deacetylase 3
HD3
SMAP45
基本信息
NACRES | NA.32 |
General description【一般描述】 | Complex of human HDAC3, GenBank Accession No. NM_003883, full length with C-terminal His tag, MW = 49.7 kDa. Human NCOR2, amino acid 395-489, GenBank Accession No. NM_006312, N-terminal ST tag, MW = 37.6 kDa, co-expressed in baculovirus expression system. |
Application【应用】 | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Unit Definition【单位定义】 | One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37℃. |
Physical form【外形】 | Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 20 mM glutathione and 10% glycerol. |
Preparation Note【制备说明】 | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles. |
产品性质
biological source【生物来源】 | human |
recombinant【重组】 | expressed in baculovirus infected insect cells |
Assay【测定】 | ≥90% (SDS-PAGE) |
form【形式】 | aqueous solution |
mol wt【分子量】 | 37.6 kDa (NCOR2) 49.7 kDa (HDAC3) |
packaging【包装】 | pkg of 50 μg |
storage condition【储存条件】 | avoid repeated freeze/thaw cycles |
concentration【浓度】 | >0.02 mg/mL |
NCBI accession no.【NCBI登记号】 | NM_003883 |
UniProt accession no.【UniProt登记号】 | O15379 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −70℃ |
Gene Information | human ... HDAC3(8841) |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 3 |