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Human CRISPR Poison Exon Knockout Library
产品性质
| packaging【包装】 | pkg of 5 vials (5x200µL aliquots ) |
| concentration【浓度】 | ≥5x108 VP/ml (via p24 Assay) |
| application(s) | CRISPR |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
基本信息
| General description【一般描述】 | The human, paired guide Poison (pgPoison) library targets 3` splice sites with paired guide RNAs for alternative exon removal (pgRNAs), induce skipping of "poison" cassette exons and corresponding upstream constitutive exons in ultraconserved regions. This compact library (targets 963 exons) allows direct comparison between loss of a constitutive coding exon (knockout) with loss of a poison exon. Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative. |
| Application【应用】 |
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| Features and Benefits【特点和优势】 |
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| Principle【原理】 | In traditional CRISPR KO screens, Cas9 introduces double-strand breaks at locations specified by a gRNA. In this library, a CRISPR-Cas9-based method is used by expressing two sgRNAs to manipulate isoforms independent of gene inactivation. This approach enables rapid suppression of exon recognition in polyclonal settings to identify functional roles for individual exons. |
| Legal Information【法律信息】 | LENTIVIRAL, WPRE AND EVROGEN FLUOROPHORE LICENSES |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
