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JMJD3/KDM6B Active human
| 产品编号： | 4119620 |
| 规格： | recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE) |
| 包装规格： | 20 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
JMJD3/KDM6B Active human
lysine (K)-specific demethylase 6B
JmjC domain-containing protein 3
KDM6B
KIAA0346
基本信息
| NACRES | NA.32 |
| General description【一般描述】 | KDM6B (lysine demethylase 6B)/ JMJD3 (jumonji domain-containing 3) is a major histone demethylase protein. This protein contains a JmjC domain in its C-terminal. Human JMJD3/KDM6B histone demethylase (amino acids 1043-end), Genbank Accession No. NM_001080424, with C-terminal FLAG-tag, MW = 71 kDa, expressed in Sf9 cells via a baculovirus expression system. |
| Application【应用】 | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Biochem/physiol Actions【生化/生理作用】 | KDM6B (lysine demethylase 6B) is responsible for the demethylation of histone H3K27. At promoters it demethylates the repressive H3K27me3 and therefore, promotes gene expression. In human and mouse fibroblasts, it demethyates the H3K27me3 at INK4a/ARF (alternate reading frame) locus, thus, promoting the expression of p16 protein, and therefore, acts as a tumor suppressor. It is also involved in inflammatory pathways in macrophages and embryonic stem cell differentiation. The expression of KDM6B is elevated in clear cell renal cell carcinoma (ccRCC) where it promotes EMT (epithelial to mesenchymal transition). Increased expression is also linked positively with lymph node metastasis, tumor size, cancer stage and poor prognosis. |
| Physical form【外形】 | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 10% glycerol and 3 mM DTT. |
| Preparation Note【制备说明】 | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles. |
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in baculovirus infected insect cells |
| Assay【测定】 | ≥70% (SDS-PAGE) |
| form【形式】 | aqueous solution |
| mol wt【分子量】 | 71 kDa |
| packaging【包装】 | pkg of 20 μg |
| NCBI accession no.【NCBI登记号】 | NM_001080424 |
| UniProt accession no.【UniProt登记号】 | O15054 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... KDM6B(23135) |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
