KRAS (Mature Form), Active

expressed in E. coli

70-95% (SDS-PAGE)

frozen liquid

2.0-3.5 nmol/min·mg (GTPase-Glo)

~23 kDa

0.1 μg/μL

NM_033360

dry ice

−70℃

human ... KRAS(3845)

Useful for enzyme assay and Western blot.

Recombinant human KRAS (2-186) was expressed in E.coli cells using an N-terminal His tag. The RAS gene superfamily encodes a group of closely related 21,000 dalton (p21) proteins with special affinity for guanine nucleotides (GTP). RAS and several other cellular proteins with similar biochemical properties are collectively known as G-proteins and they play key roles in a wide variety of cellular activities, including cell growth, differentiation, secretion, and protein trafficking ⁽¹⁾. There are three forms of RAS gene in cells termed H-RAS, N-RAS, and K-RAS. RAS proteins play a direct causal role in human cancer and in other diseases. Mutant H-RAS, N-RAS, and K-RAS occur in varying frequencies in different tumor types ⁽²⁾. Other members of the RAS superfamily may also contribute to cancer.

Formulated in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.

PRECISIO is a registered trademark of sigma-aldrich Co. LLC

Store in aliquots at -70℃. Avoid freeze/thaw cycles.

GHS07,GHS08

Danger

H315 - H319 - H360D

P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK 1