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L-Lactate Assay Kit

产品编号:4119728
规格:Sufficient for 100 fluorometric tests
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4119728Sufficient for 100 fluorometric tests4870
产品性质
Quality Level【质量水平】
100
Detection Method
fluorometric
relevant disease(s)【相关疾病】
diabetes; pulmonary disorders; cardiovascular diseases; cancer; endocrinological disorders
storage temp.【储存温度】
−20℃
suitability【适用性】
The kit is suitable for the quantitative determination of L-lactate (L-lactic acid) and the evaluation of drug effects on its metabolism in serum, plasma, urine, cell culture media, and other biological samples.
基本信息
General description【一般描述】
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.
Features and Benefits【特点和优势】
  • Detection limit of 1 μM and linearity up to 50 μM L-lactate in 96-well plate assay.
  • Convenient room temperature assay. The procedure involves adding a single working reagent, and reading the fluorescence after 60 minutes.
  • Compatible with high-throughput handling systems.
Principle【原理】
The L-Lactate Assay Kit is based on the lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λEx = 530 nm/λEm = 585 nm, is proportional to the lactate concentration in the sample.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 2
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