LEPR/CD295 human

NA.32

Leptin receptor (LEPR), also known as LEP-R, cluster of differentiation 295 (CD295), OB-R and B219, is a single-transmembrane-domain receptor of the gp130 family of cytokine receptors. Leptin receptor exists as homodimer and binds Leptin with high affinity, thus mediates the biological function of the adipocyte-specific hormone Leptin. LEPR is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this protein have been associated with obesity and pituitary dysfunction. Interaction of leptin and leptin receptor is crucial for body weight and bone mass regulation in mammals through hypothalamic effects on satiety and energy expenditure. Meanwhile, research data supports a leptin receptor activation model based on ligand-induced conformational changes.

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8℃ for up to 1 month. For extended storage, it is recommended to store at -20℃.

human

expressed in HEK 293 cells

≥95% (SDS-PAGE)

lyophilized

calculated mol wt 121 kDa (This protein is fused with Fc fragment of human IgG1 at the C-terminus.)
observed mol wt 150-165 kDa by SDS-PAGE (reducing) (Phe22 is the predicted N-terminal.)

pkg of 10 and 50 μg

<1 EU/μg endotoxin (LAL test)

P48357

wet ice

−20℃

human ... LEPR(3953)

11 - Combustible Solids

WGK 3