您的位置：首页 > 产品中心 > MEK5, Active
MEK5, Active
产品性质
| recombinant【重组】 | expressed in baculovirus infected Sf9 cells |
| Assay【测定】 | 0.05-0.1 μg/μL 70-95% (SDS-PAGE) |
| form【形式】 | frozen liquid |
| Specific Activity | 70-100 nmol/min·mg (radiometric) |
| mol wt【分子量】 | ~77 kDa |
| accession no.【登记号】 | BC008838 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... MEK5(5607) |
基本信息
| Application【应用】 | Useful for kinase assay and Western blot. |
| Biochem/physiol Actions【生化/生理作用】 | Recombinant full-length human MEK5 was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. MEK5 or MAP2K5 (mitogen-activated protein kinase kinase 5) is a dual specificity protein kinase that belongs to the MAP kinase kinase family which specifically interacts with and activates MAPK7/ERK5. MEK5 itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by MEK5 is involved in growth factor stimulated cell proliferation and muscle cell differentiation (1). The rs3743354 polymorphism in the MEK5 promoter may affect the risk of developing colorectal cancer (CRC) (2). |
| Reconstitution【重悬】 | Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol. |
| Legal Information【法律信息】 | PRECISIO is a registered trademark of sigma-aldrich Co. LLC |
产品说明
| Storage and Stability【储存及稳定性】 | Store in aliquots at -70℃. Avoid freeze/thaw cycles. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
