Milli-Mark^® Anti-CD27-FITC Antibody, clone M-T271

32160702

NA.44

CD27, a member of the nerve growth factor receptor (NGFR) superfamily, is a type I transmembrane protein consisting of a disulphide-linked 120 kDa homodimer (Bigler, 1988; Sugita, 1992; Morimoto, 1995). CD27 is expressed on mature thymocytes and on the majority of human peripheral blood T cells, on both CD4+ and CD8+ subsets. CD27 is also expressed on activated B cells and a fraction of resting natural killer (NK) cells (Maurer, 1990; Sugita, 1992). Among CD4+ T cells, CD27 is preferentially expressed on unprimed CD4+CD45RA+CD45R0- T cells, while primed CD4+CD45RA-CD45R0+ T cells express low levels of CD27 (Sugita, 1992). During activation, the expression of CD27 is increased on B cells and unprimed T cells, while primed T cells have a relatively low capacity for upregulation of CD27 (Morimoto, 1995; Hintzen, 1994). The activation is also accompanied by the appearance of a 32 kDa soluble form of CD27 (sCD27) found in supernatants of activated lymphocytes (Hintzen, 1994).
CD27 is believed to play a role in T cell activation, since the expression is upregulated during T cell activation (Bigler, 1988; Sugita, 1992; Morimoto, 1995; Maurer, 1990; Hintzen, 1994; Lens, 1995).

Antibody recognizes Human CD27 antigen.

Leukemic peripheral blood T cells from a patient with chronic T cell leukemia (T-CLL)

Research Sub Category
Immunoglobulins & Immunology
This Milli-Mark Anti-CD27-FITC Antibody, clone M-T271 is validated for use in FC for the detection of CD27.
Research Category
Inflammation & Immunology

Evaluated by flow cytometry using human PBMCs

Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Protein A purified

Control
PBMCs

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

FITC conjugate

purified antibody

primary antibodies

M-T271, monoclonal

human

Milli-Mark^®

flow cytometry: suitable

IgG1κ

NP_001233.1

P26842

wet ice

120 kDa (Bigler, 1988; Sugita, 1992; Morimoto, 1995)

Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2