PFTK1 (CDK14)/CYCLINY, Active

expressed in baculovirus infected Sf9 cells

70-95% (SDS-PAGE)

frozen liquid

2.0-3.5 nmol/min·mg (radiometric)

~64 kDa (Cyclin Y)
~72 kDa (PFTK1 (CDK14))

0.1 μg/μL

NM_012395
NM_145012

dry ice

−70℃

human ... CCNY(219771), CDK14(5218)

Useful for Kinase assay and Western blot.

Recombinant human full-length PFTK1 (CDK14) and Cyclin Y (2-end) were co-expressed by Baculovirus in Sf9 insect cells using N-terminal GST tags. PFTK1 is a member of the CDC2 - related protein kinase family which is expressed primarily in the postnatal and adult nervous system ⁽¹⁾. PFTK1 is expressed at high levels in brain, pancreas, kidney, heart, testis, and ovary. PFTK1 amino acid residue ser119 is required for its interaction with all four 14-3-3 isoforms, while ser120 may contribute slightly to PFTK1 binding ⁽²⁾. PFTK1 acts as a cyclin-dependent kinase that regulates cell cycle progression and cell proliferation. Overexpression of PFTK1 predicts resistance to chemotherapy in patients with oesophageal squamous cell carcinoma.

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

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Store in aliquots at -70℃. Avoid freeze/thaw cycles.

10 - Combustible liquids

WGK 1