PhosphoDetect Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

liquid

preservative

human, mouse, rat

Calbiochem^®

OK to freeze

IgG

wet ice

−20℃

50 μL in Plastic ampoule

This PhosphoDetect Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb is validated for use in Immunoblotting , Immunoprecipitation for the detection of p53 (pSer¹⁵) (Ab-3).
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa p53 protein phosphorylated on Ser¹⁵.


Recognizes the ~53 kDa p53 protein phosphorylated at Ser¹⁵ in PC12 or HeLa cells treated with DNA damaging agents. Does not recognize unphosphorylated p53.

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵ phosphorylation site of human p53, conjugated to KLH

Immunoblotting (1:1000)
Immunoprecipitation (1:200)

Toxicity: Standard Handling (A)

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Following initial thaw, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.

Positive Control
PC-12, HCT116, or HeLa cells treated with DNA damaging agents

Does not recognize non-phosphorylated p53. Antibody should be titrated for optimal results in individual systems.
Tibbetts, R.S., et al. 1999. Genes Dev.13, 152.
Levine, A.J. 1997. Cell88, 323.
Milczarek, G.J., et al. 1997. Life Sci.60, 1.
Shieh, S.Y., et al. 1997. Cell91, 325.
Meek, D.W. 1994. Semin Cancer Biol.5, 203.

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