PIP5K3 (PIKFYVE), Active

expressed in baculovirus infected Sf9 cells

70-95% (SDS-PAGE)

frozen liquid

90-115 nmol/min·mg (ADP-Glo)

~110 kDa

0.05-0.1 μg/μL

NM_015040

dry ice

−70℃

human ... PIP5K3(200576)

Useful for kinase assay.

Recombinant human PIP5K3 (1400-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. PIP5K3 (also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) encodes an enzyme that phosphorylates the D-5 position in PtdIns and phospha-tidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns (3,5)biphosphate. PIP5K3 belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol but also have protein kinase activity ⁽¹⁾. PIP5K3 regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. The inhibition of PIP5K3 renders the late endosome /lysosome compartment refractory to fusion with autop-hagosomes and multivesicular bodies ⁽²⁾.

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

PRECISIO is a registered trademark of sigma-aldrich Co. LLC

Store in aliquots at -70℃. Avoid freeze/thaw cycles.

10 - Combustible liquids

WGK 1