PLK4, active, GST tagged human

NA.32

PLK4 or polo-like kinase 4 is a member of the polo family of serine/threonine protein kinases which localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. The overexpression of PLK4 triggered the simultaneous formation of multiple procentrioles around each preexisting centriole which resulting in centriole amplification and thus, Plk4-induced centriole biogenesis in human cells.The reduced Plk4 gene dosage increases the probability of mitotic errors and cancer development.

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

After opening, aliquot into smaller quantities and store at -70 ℃. Avoid repeating handling and multiple freeze/thaw cycles.

PRECISIO is a registered trademark of sigma-aldrich Co. LLC

human

expressed in baculovirus infected Sf9 cells

PRECISIO^®

≥70% (SDS-PAGE)

buffered aqueous glycerol solution

4.5-6.7 nmol/min·mg

~140 kDa

NM_014264

cell analysis

dry ice

−70℃

human ... PLK4(10733)

10 - Combustible liquids

WGK 1