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pNPP Phosphatase Assay Kit

产品编号:4139809
规格:Sufficient for 500 colorimetric tests
包装规格:1 kit
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
41398091 kit3040
产品性质
Quality Level【质量水平】
100
Detection Method
colorimetric
storage temp.【储存温度】
−20℃
suitability【适用性】
The kit is suitable for the quantitative determination of phosphatase activity of protein phosphatases.
基本信息
General description【一般描述】
Para-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at A405 nm on a spectrophotometer.
Features and Benefits【特点和优势】
  • No wash or reagent transfer steps are involved. The assay can be completed within 30 minutes.
  • Compatible with high-throughput handling systems.
Principle【原理】
The pNPP Phosphatase Assay kit involves adding a single reagent to the phosphatase and measuring the product formation on an absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37 ℃.
安全信息
Pictograms【象形图】
GHS05,GHS07,GHS08,GHS09
Signal word【警示用语:】
Danger
Hazard Statements
H302 + H332 - H314 - H341 - H373 - H411
Precautionary Statements
P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
Hazard Classifications【危险分类】
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
Storage Class Code【储存分类代码】
8A - Combustible, corrosive hazardous materials
WGK
WGK 3
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