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PRMT5/MEP50 Active human
| 产品编号： | 4139946 |
| 规格： | recombinant, expressed in FreeStyleTM 293-F cells, ≥60% (SDS-PAGE) |
| 包装规格： | 20 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
PRMT5/MEP50 Active human
HMT1 hnRNP methyltransferase-like 5(HRMT1L5)
shk1 kinase-binding protein 1 homolog (SKB1)
Jak-binding protein 1 (JBP1)
ICln-binding protein (IBP72) 72 kDa
Arginine methyltransferase 5
基本信息
| NACRES | NA.32 |
| General description【一般描述】 | Protein arginine methyltransferase 5 (PRMT5) and WD repeat-containing protein (WDR77) or methylosome protein- 50 (MEP-50) are part of the methylosome. PRMT5 is a type II PRMT enzyme. The gene encoding it is localized to human chromosome 14q11.2. Complex of human PRMT5 (GenBank Accession No. NM_006109), amino acid 2-end, with N-terminal FLAG®-tag, MW=73 kDa, and human MEP50 (GenBank Accession No. NM_024102), amino acid 2-end, with N-terminal His tag, MW=37.5 kDa, expressed in FreeStyle 293-F cells. |
| Application【应用】 | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Recombinant PRMT5/MEP50 has been used in the detection of arginine methylation in in vitro methyltransferase assay. |
| Biochem/physiol Actions【生化/生理作用】 | Methylosome protein 50 (MEP50) binds to the oncogenic protein arginine methyltransferase 5 (PRMT5) and forms a complex called methylosome. This complex is involved in mono- and symmetric dimethylation of arginine, which is necessary for various biological processes. MEP50 acts as a coactivator for androgen receptor (AR) and estrogen receptor (ER). PRMT5 also methylates histones and other proteins involved in genome organization, transcription, stem cells, primordial germ cells, differentiation, the cell cycle, and spliceosome assembly. The two proteins have been associated with cancer tumorigenesis and are marked as potential oncogenes. |
| Unit Definition【单位定义】 | One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37℃. |
| Physical form【外形】 | Formulated in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 80 μg/ml FLAG® peptide, and 20% glycerol. |
| Preparation Note【制备说明】 | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles. |
| Legal Information【法律信息】 | FLAG is a registered trademark of sigma-aldrich Co. LLC FreeStyle is a trademark of Invitrogen Corp. |
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in FreeStyle™ 293-F cells |
| Assay【测定】 | ≥60% (SDS-PAGE) |
| form【形式】 | aqueous solution |
| mol wt【分子量】 | 37.5 kDa (MEP50) 73 kDa (PRMT5) |
| packaging【包装】 | pkg of 20 μg |
| manufacturer/tradename | sigma-aldrich |
| storage condition【储存条件】 | avoid repeated freeze/thaw cycles |
| concentration【浓度】 | >0.02 mg/mL |
| NCBI accession no.【NCBI登记号】 | NM_006109 (PRMT5) NM_024102 (MEP50) |
| UniProt accession no.【UniProt登记号】 | O14744 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... PRMT5(10419) |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 3 |
