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RAD51 人

RAD51 human
产品编号:4140220
规格:recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
包装规格:10 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
414022010 μG5410
产品别名

RAD51 人

RAD51 human

BRCC5

HsT16930

RAD51A

HsRad51

HRAD51

RECA

基本信息
NACRES
NA.26
General description【一般描述】
RAD51 is a member of the RecA/RadA/Rad51 protein family which is characterized by a conserved ATP binding core. Human RAD51 shows predominant expression in testis, ovary, and lymphoid tissue.
RAD51 gene is mapped to human chromosome 15q15.1. It is an Escherichia coli Recombinase A (RecA) homolog and comprises an N-terminal DNA binding domain.
Biochem/physiol Actions【生化/生理作用】
Properly controlled recombination between homologous DNA molecules (Homologous Recombination - HR) is essential for the maintenance of genome stability and for the prevention of tumorigenesis. RAD51 is a mammalian homologue of yeast RAD51 and bacterial RecA and, like its counterparts, plays a central role in HR. RAD51 coats ssDNA to form a nucleoprotein filament that invades and pairs with a homologous region in duplex DNA. It can then activate strand exchange to generate a crossover between the juxtaposed DNA molecules. In addition to RAD51, these steps require the coordinated action of a number of other homologous-recombination proteins, including the RP-A protein, which binds single-stranded DNA, RAD52, which can bind DNA ends, anneal complementary single-stranded DNA molecules and enhance the specificity of RAD51, and a number of RAD51 paralogs. The tumour-suppressor proteins BRCA1 and BRCA2 colocalize with RAD51 in DNA-damage-induced nuclear foci. BRCA2 has been shown to interact directly with RAD51 and thus plays a direct role in repair by HR, through control of the availability and function of the central mediator, RAD51.
RAD51 interacts with BRCA2 (breast cancer susceptibility protein) and participates in DSB (double strand DNA breaks) repair. BRCA2 sequesters and recruits RAD51 to the site of the damage and promotes the formation of the helical RAD51–single stranded DNA (ssDNA) nucleoprotein filaments. These filaments look for and infiltrate the homologous DNA template, and promote homologous recombination by inducing DNA polymerization and strand exchange. A dominant-negative mutation in this gene is linked with a novel Fanconi anaemia (FA) subtype, a disorder characterized by developmental abnormalities, bone marrow failure and a strong susceptibility to cancer. Association of RAD51 gene with congenital mirror movement disorders signifies the importance of RAD51-mediated homologous recombination in neurodevelopment apart from in DNA repair, cancer susceptibility.
Physical form【外形】
Clear and colorless frozen liquid solution
Preparation Note【制备说明】
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
产品性质
biological source【生物来源】
human
recombinant【重组】
expressed in E. coli
Assay【测定】
≥80% (SDS-PAGE)
form【形式】
frozen liquid
mol wt【分子量】
~39.1 kDa
packaging【包装】
pkg of 10 μg
storage condition【储存条件】
avoid repeated freeze/thaw cycles
concentration【浓度】
500 μg/mL
technique(s)
electrophoretic mobility shift assay: suitable
color【颜色】
clear colorless
NCBI accession no.【NCBI登记号】
NM_002875
UniProt accession no.【UniProt登记号】
Q06609
shipped in【运输】
dry ice
storage temp.【储存温度】
−70℃
Gene Information
human ... RAD51(5888)
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 1
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