SERPINA8 human

NA.32

Serpin A8 also known as angiotensinogen (AGT) , is a member of the serpin family. It is an α-2-globulin that is expressed by the liver and secreted in plasma. As essential component of the renin-angiotensin system (RAS), Angiotensinogen is cleaved into three chains: Angiotensin-1 (Ang I), Angiotensin-2 (Ang II), and Angiotensin-3 (Ang III) in lowered blood pressure by the enzyme renin. Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Angiotensin-3 stimulates aldosterone release. Defects in AGT are a cause of renal tubular dysgenesis (RTD).

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8℃ for up to 1 month. For extended storage, it is recommended to store at -20℃.

human

expressed in HEK 293 cells

6-His tagged (N-terminus)

≥98% (SDS-PAGE)

lyophilized powder

calculated mol wt 50.6 kDa
observed mol wt 55-60 kDa (DTT-reduced. Protein migrates due to glycosylation. Asp 34 is the predicted N-terminus.)

pkg of 10 μg

<1 EU/μg endotoxin (LAL test)

P01019

wet ice

−20℃

human ... AGT(183)

11 - Combustible Solids

WGK 3