活性 SHP-1 人

NA.32

The Src homology region 2 domain-containing phosphatase-1 (SHP1)/protein tyrosine phosphatase, non-receptor type 6 (PTPN6) is a 68 kDa protein present in the cytoplasm. It is mapped to human chromosome 12p13.
Protein tyrosine phosphatase, full length, (Gene bank accession number NM_080549) with N-terminal GST tag, MW = 95 kDa, expressed in an E. coli expression system.

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

In mammary epithelial cells, protein tyrosine phosphatase, non-receptor type 6 (PTPN6) controls EGFR (epidermal growth factor receptor) signaling. It participates in the JAK/STAT (janus kinase/signal transducers and activators of transcription) signaling pathway. PTPN6 regulates cytokine receptors like interleukin- 3 (IL-3) and erythropoietin.

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30℃.

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

human

expressed in E. coli

≥90% (SDS-PAGE)

aqueous solution

95 kDa

pkg of 20 μg

avoid repeated freeze/thaw cycles

1 mg/mL

NM_080549

P29350

dry ice

−70℃

human ... PTPN6(5777)

10 - Combustible liquids

WGK 1