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SMURF1 Active human
| 产品编号： | 4140622 |
| 规格： | recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE) |
| 包装规格： | 20 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
SMURF1 Active human
KIAA1625
SMAD specific E3 ubiquitin protein ligase 1
E3 ubiquitin ligase SMURF1
基本信息
| NACRES | NA.32 |
| General description【一般描述】 | Human SMURF 1 (GenBank Accession No. NM_020429), amino acids 150-end, with N-terminal DDDDK tag (FLAG) MW = 70 kDa, expressed in a Baculovirus infected Sf9 cell expression system. |
| Application【应用】 | Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Unit Definition【单位定义】 | One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37℃. |
| Physical form【外形】 | Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT. |
| Preparation Note【制备说明】 | Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles. |
产品性质
| biological source【生物来源】 | human |
| recombinant【重组】 | expressed in baculovirus infected insect cells |
| Assay【测定】 | ≥80% (SDS-PAGE) |
| form【形式】 | aqueous solution |
| Specific Activity | ≥0.0055 pmol/min-μg |
| mol wt【分子量】 | 70 kDa |
| packaging【包装】 | pkg of 20 μg |
| storage condition【储存条件】 | avoid repeated freeze/thaw cycles |
| concentration【浓度】 | >0.02 mg/mL |
| NCBI accession no.【NCBI登记号】 | NM_020429 |
| UniProt accession no.【UniProt登记号】 | Q9HCE7 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | human ... SMURF1(57154) |
