SMURF2 Active human

NA.32

Human SMURF 2 (GenBank Accession No. NM_022739), amino acids 130-end, with N-terminal DDDDK tag (FLAG), MW = 72 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37℃.

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

human

expressed in baculovirus infected insect cells

≥80% (SDS-PAGE)

aqueous solution

≥0.00049 pmol/min-μg

72 kDa

pkg of 20 μg

avoid repeated freeze/thaw cycles

>0.02 mg/mL

NM_022739

Q9HAU4

dry ice

−70℃

human ... SMURF2(64750)