SMYD2 Active human

NA.32

SET and MYND domain containing 2 (SMYD2) is encoded by the gene mapped to human chromosome 1q32.3-q41. The encoded protein belongs to SMYD family of protein lysine methyltransferases.
Human SMYD2 (GenBank Accession No. NM_020197), full length with N-terminal DDDDK tag (FLAG), MW = 49.7kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

SET and MYND domain containing 2 (SMYD2) might act as a putative oncogene by methylating p53 and reducing its tumor suppressive role. Overexpression of SMYD2 gene is associated with pathogenesis and metastasis of oesophageal cancer, squamous cell carcinoma (ESCC). Therefore, it is considered as a potent therapeutic target for ESCC. SMYD2 has capacity to catalyze the methylation of histone and non-histone substrates. AZ505 acts as a potent and selective inhibitor of SMYD2 and aids in the improvement of novel cancer treatment.

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

human

expressed in baculovirus infected insect cells

≥80% (SDS-PAGE)

aqueous solution

49.7 kDa

pkg of 20 μg

avoid repeated freeze/thaw cycles

>0.02 mg/mL

NM_020197

Q9NRG4

dry ice

−70℃

human ... SMYD2(56950)

10 - Combustible liquids

WGK 1