您的位置：首页 > 产品中心 > TRXB from Escherichia coli
TRXB from Escherichia coli
| 产品编号： | 4141224 |
| 规格： | recombinant, expressed in E. coli, ≥90% (SDS-PAGE) |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
TRXB from Escherichia coli
TRXR
Thioredoxin reductase
基本信息
| NACRES | NA.32 |
| General description【一般描述】 | Thioredoxin reductase (TrxR) is encoded by TRXB gene. TrxR is one of the member of flavoprotein family of pyridine nucleotide-disulphide oxidoreductases, which also includes lipoamide dehydrogenase, glutathione reductase and mercuric ion reductase. Escherichia coli TrxR is a 70-kDa homodimeric flavoprotein, each monomer of this protein contains a FAD prosthetic group, an NADPH domain and an active site with redox-active disulphide. The catalytic site (-Cys-Ala-Thr-Cys-) in Escherichia coli TrxB is localized on the NADPH domain, whereas in humans TrxB catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) is part of the FAD domain. |
| Biochem/physiol Actions【生化/生理作用】 | Thioredoxin reductase (TRXB) catalyzes the reduction of oxidized thioredoxin using nicotinamide adenine dinucleotide phosphate (NADPH). In thioredoxin pathway, this reduced thioredoxin reduce disulfide bonds in proteins localized in the cytoplasm and is implicated in the recycling of an essential enzyme ribonucleotide reductase. TrxR is essentially involved in protection against oxidation stress, cell growth and transformation, and the recycling of ascorbate from its oxidized form. |
| Physical form【外形】 | 1 mg/mL solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol and 1mM DTT. |
| Preparation Note【制备说明】 | Centrifuge the vial prior to opening. |
| Other Notes【其他说明】 | MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K. |
产品性质
| biological source【生物来源】 | Escherichia coli |
| recombinant【重组】 | expressed in E. coli |
| Assay【测定】 | ≥90% (SDS-PAGE) |
| form【形式】 | liquid |
| mol wt【分子量】 | 34.6 kDa |
| packaging【包装】 | pkg of 100 μg |
| NCBI accession no.【NCBI登记号】 | P0A9P4 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | Escherichia coli ... TRXB(949054) |
安全信息
| Pictograms【象形图】 | GHS07 |
| Signal word【警示用语：】 | Warning |
| Hazard Statements | H319 |
| Precautionary Statements | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| Hazard Classifications【危险分类】 | Eye Irrit. 2 |
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 3 |
