Anti-c-ErbB2/c-Neu (Ab-5) Mouse mAb (TA-1)

100

mouse

purified antibody

primary antibodies

TA-1, monoclonal

liquid

≤0.1% sodium azide as preservative

human

rat, mouse

Calbiochem^®

do not freeze

IgG1

wet ice

2-8℃

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing spleonocytes with SP2/0 mouse myeloma cells (see application references). Recognizes the c-ErbB2/c-neu protein.
Anti-c-ErbB2/c-Neu (Ab-5), mouse monoclonal, clone TA-, recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells. It is validated for FC, WB, IF, IP, and IHC on frozen sections.

Flow Cytometry (5 µg/ml, see application references)

Frozen Sections (5 µg/ml)

Immunoblotting (see application references)

Immunofluorescence (5 µg/ml, see application references)

Immunoprecipitation (1 µg/sample, see application references)

Neutralization Studies (not recommended, see comments and application references)

Toxicity: Standard Handling (A)

In 50 mM PBS, 0.2% gelatin, pH 7.5.

Positive Control
SK-BR-3 cells

Recognizes an extracellular epitope and does not cross react with human EGF-receptor. It will not inhibit in vivo protein tyrosine kinase activity. Antibody should be titrated for optimal results in individual systems.
DiFiore, P.P., et al. 1987. Science237, 178.
Slamon, D.J., et al. 1987. Science235, 177.
Varley, J.M., et al. 1987. Oncogene1, 423.
Yamamoto, T., et al. 1986. Nature319, 230.
Blick, M., et al. 1984. Blood64, 1234.
Schwab, M., et al. 1984 Cold Spring Harbor Laboratory2, 215.
Bargmann, C.I., et al. 1968. Nature319, 226.

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11 - Combustible Solids

WGK 1