Anti-Cathepsin D (Ab-1) Mouse mAb (BC011)

100

mouse

purified antibody

primary antibodies

BC011, monoclonal

liquid

≤0.1% sodium azide as preservative

human

Calbiochem^®

do not freeze

IgG2a

wet ice

2-8℃

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Recognizes the ~52 kDa latent form, the ~48 kDa mature form, and the ~34 kDa subunit of cathepsin D in breast carcinoma tissue extracts.
Purified mouse monoclonal antibody generated by immunizing CB6F1 mice with the specified immunogen and fusing splenocytes with SP2/0 cells. Recognizes the ~52 kDa pro-cathespin D, the ~48 kDa mature cathepsin D, and the ~34 kDa subunit of cathespin D.
Anti-Cathepsin D (Ab-1), mouse monoclonal, clone BC011, recognizes latent and mature form and the ~34 kDa subunit in breast carcinoma tissue extracts. It is validated for WB and for paraffin sections.

mature, human cathepsin D

Immunoblotting (1 µg/ml)

Paraffin Sections (5 µg/ml, trypsin pre-treatment required)

Toxicity: Standard Handling (A)

Rochefort, H. 1992. Eur. J. Cancer.28A, 1780.
Rochefort, H. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 153.
Westly, B. and Rochefort, H. 1980. Cell20, 352.
Barrett, A.J. 1977. in Proteinases in Mammalian Cells and Tissues, ed. Barret, A.J. (North Holland, New York), pp. 209.
Does not detect the ~14 kDa subunit of cathespin D, nor will it detect cathespins B or L. Cathepsins are secreted and accumulate in conditioned tissue culture media and can be detected by immunoblotting from this source. Some cell extracts do not contain sufficient cathepsin for detection by immunoblotting. Antibody should be titrated for optimal results in individual systems.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

11 - Combustible Solids

WGK 1