Anti-CD64 Mouse mAb (10.1)

100

mouse

purified antibody

primary antibodies

10.1, monoclonal

≥95% (SDS-PAGE)

liquid

0.5 mg/mL gentamycin sulfate as preservative

human

Calbiochem^®

OK to freeze

IgG1

wet ice

2-8℃

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Recognizes the ~72 kDa CD64 protein (FcR1 receptor).
Anti-CD64, mouse monoclonal, clone 10.1, recognizes the ~72 kDa CD64 protein (FcR1). It is validated for FC, WB, IP, IHC on frozen sections, and for neutralization studies.
Purified mouse monoclonal antibody. Recognizes the ~72 kDa CD64 (FcR1) protein.

human rheumatoid synovial fluid cells/monocytes
Human

Flow Cytometry (10 µg/ml)

Frozen Sections (see comments)

Immunoblotting (see comments)

Immunoprecipitation (see comments)

Neutralization Studies (see comments)

Toxicity: Standard Handling (A)

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Negative Control
HPB-MLT
Positive Control
4937 cells

The antibody is reported to block immunoglobulin binding to FcR1. FACS was performed on 5x10⁵ cultured U-937 human tumor cells and analyzed by flow cytometry. Optimal binding concentration tested was 10 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Dougherty, G.J., et al. 1987. Eur. J. Immunol.17, 1453.

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10 - Combustible liquids

WGK 1