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Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)
产品别名
Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)
Anti-Epidermal Growth Factor Receptor
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | EGFR.1, monoclonal |
| form【形式】 | liquid |
| contains【包含】 | ≤0.1% sodium azide as preservative |
| species reactivity | human |
| should not react with | rat, mouse |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | do not freeze |
| isotype【同位素/亚型】 | IgG2b |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | 2-8℃ |
| packaging【包装】 | 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
| General description【一般描述】 | Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein. Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, and IP. Recognizes the ~170 kDa EGF receptor protein in A431 cells. For neutralization studies Cat. No. GR15L is recommended. |
| Immunogen【免疫原】 | partially purified, human EGFR from A431 cells Human |
| Application【应用】 |  Flow Cytometry (1-5 g/ml) Immunofluorescence (1-5 g/ml) Immunoprecipitation (1-2 g/sample) Neutralization Studies (1-2 g/ml; use Cat. No. GR15L; see application references) |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Physical form【外形】 | In 50 mM sodium phosphate, 0.2% gelatin, pH 7.5. |
| Analysis Note【分析说明】 | Negative Control HL-60 cells Positive Control A-431 cells |
| Other Notes【其他说明】 | EGF-Receptor (Ab-5) binds to A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems. Hunter T., 1984. Nature311, 414. Reynolds, F.H., Jr., et al. 1981. Nature292, 259. Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | nwg |
