Anti-IRP2 Antibody, clone 3B11

32160702

Iron-responsive element-binding protein 2 (UniProt: P48200; also known as IRE-BP 2, Iron regulatory protein 2, IRP2) is encoded by the IREB2 gene (Gene ID: 3658) in human. IRP2 is a member of the aconitase/IPM isomerase family that serves as an RNA-binding protein, which regulates the homeostatic binding of intracellular iron to its specific cognate mRNA hairpin structures known as iron-responsive elements (IREs). Its interaction with IRE is shown to be abolished under conditions of high iron. In iron rich cells and in the presence of oxygen, IRP2 is ubiquitinated and degraded in proteasome pathway. Its ubiquitination is achieved by a SCF complex containing FBXL5. Upon iron and oxygen depletion FBXL5 is degraded, preventing ubiquitination and allowing its RNA-binding activity. IN IRP2, Cys201 and His204 have been shown to be critical for IRP2 degradation. Cys201 binds ferric heme and His204 binds ferrous heme and they are involved in sensing the redox state of the heme iron and in generation the oxidative modification. Two isoforms of IRP2 have been described that are produced by alternative splicing. (Ref.: Ashizuka, M., et al. (2002). Mol. Cell. Biol. 22(18); 6375-6383; Ishikawa, H., et al. (2005). Mol. Cell 19(2); 171-181).

Clone 3B11 is a mouse monoclonal antibody that detects Iron regulatory protein 2 (IRP2). It targets an epitope with in 73 amino acids from the N-terminal region.

GST-tagged recombinant fragment corresponding to 73 amino acids from the N-terminal region of human Iron regulatory protein 2 (IRP2).

Anti-IRP2, clone 3B11 , Cat. No. MABS2030, is a mouse monoclonal antibody that detects Iron-responsive element-binding protein 2 and has been tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Research Category
Signaling
Immunoprecipitation Analysis: A representative lot immunoprecipitated IRP2 in Immunoprecipitation applications (Ashizuka, M., et. al. (2002). Mol Cell Biol. 22(18):6375-83).

Western Blotting Analysis: A representative lot detected IRP2 in Western Blotting applications (Yoshinaga, M., et. al. (2017). Cell Rep. 19(8):1614-1630).

Evaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected IRP2 in human kidney tissue sections.

Protein G purified
Purified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Concentration: Please refer to lot specific datasheet.

mouse

purified immunoglobulin

primary antibodies

3B11, monoclonal

human, mouse

antibody small pack of 25 μg

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

IgGκ

NP_001307870

P48200

human ... IREB2(3658)

105.06 kDa calculated.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.