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Anti-LDL Receptor Mouse mAb (15C8)
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | 15C8, monoclonal |
| form【形式】 | liquid |
| contains【包含】 | ≤0.1% sodium azide as preservative |
| species reactivity | bovine, human |
| should not react with | rabbit |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | do not freeze |
| isotype【同位素/亚型】 | IgG2a |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | 2-8℃ |
| packaging【包装】 | 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
| General description【一般描述】 | Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 myeloma cells (see application references). Recognizes the ~160 kDa LDL Receptor protein. Recognizes the ~160 kDa LDL receptor protein in HT1080 and HepG2 cells. This Anti-LDL Receptor Mouse mAb (15C8) is validated for use in ELISA, Flow Cytometry, Frozen Sections, Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of LDL Receptor. |
| Immunogen【免疫原】 | Bovine a trypic digest of bovine low density lipoprotein receptor Epitope: first cysteine-rich ligand binding domain |
| Application【应用】 |  ELISA (0.5-1 g/ml) Flow Cytometry (see application references) Frozen Sections (1-5 g/ml) Immunoblotting (1-5 g/ml, see application references) Immunocytochemistry (1-5 g/ml, IF) Immunoprecipitation (1-2 g/sample) |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Physical form【外形】 | In 0.05 M sodium phosphate buffer, 0.2% gelatin. |
| Analysis Note【分析说明】 | Positive Control Liver sections, HT1080 cells, HepG2 cells |
| Other Notes【其他说明】 | Will displace 50-70% of LDL binding to the receptor. For immunoblotting, include 2 mM Ca+2 with the antibody and avoid EDTA. This antibody has been reported to bind best to the unreduced LDL-R (see specificity application reference). Antibody should be titrated for optimal results in individual systems. van Driel, I.R., et al. 1987. J. Biol. Chem.262, 16127. van Driel, I.R., et al. 1987. J. Biol. Chem.262, 17443. Russell, D.W., et al. 1984. Cell37, 577. Achneider, W.J., et al. 1983. J. Cell Biol.97, 1635. Cummings, R.D., et al. 1983. J. Biol. Chem.258, 15261. Schneider, W.J., et al. 1982 J. Biol. Chem.257, 2664. Goldstein, J.L., et al. 1979. Nature279, 679. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | nwg |
