Anti-MMP-7 (Ab-1) Mouse mAb (141-7B2)

100

mouse

purified antibody

primary antibodies

141-7B2, monoclonal

liquid

≤0.1% sodium azide as preservative

human

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG1

wet ice

−20℃

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Recognizes the ~28 kDa latent form of MMP-7 in TPA-treated SW620 cells. Does not recognize the active form of MMP-7.
Purified mouse monoclonal antibody. Recognizes the ~28 kDa latent form of MMP-7.
This Anti-MMP-7 (Ab-1) Mouse mAb (141-7B2) is validated for use in Immunoblotting, Paraffin Sections for the detection of MMP-7 (Ab-1).

human rectal carcinoma MMP-7
Human

Immunoblotting (5-10 g/ml, see application references)
Paraffin Sections (see application references)

Toxicity: Standard Handling (A)

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
CaR-1 or TPA treated SW620 cells

Brunner, K.L, et al. 1995. Proc. Natl. Acad. Sci. USA92, 7362.
Imai, K., et al. 1995. J. Biol. Chem.270, 6691.
Lichtinghagen, R., et al. 1995. Eur. J. Clin. Chem. Clin. Biochem.33, 65.
Nakano, A., et al. 1995. J. Neurosurg.83, 298.
Takino, T., et al. 1995. J. Biol. Chem.270, 23013.
Woessner, J.F. 1991. FASEB J.5, 2145.
Yamamoto, H., et al. 1995. J. Clin. Lab. Anal.9, 297.
Gaire, M., et al. 1994. J. Biol. Chem.269, 2032.
McDonnell, S., et al. 1994. Biochem. Soc. Trans.22, 58.
Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol.2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J.7, 1434.
Woessner, J.F. 1991. FASEB J.5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Woessner, J.F., Jr and Taplin, C.J. 1988. J. Biol. Chem.263, 16918.
Sellers, A. and Woessner, J.F., Jr. 1980. Biochem. J.189, 521.
Does not recognize the ~19 kDa active form of MMP-7. To detect active MMP-7, use Cat. No. IM47L. Does not cross-react with human MMP-1, MMP-2, MMP-3, MMP-8, MMP-9 or MMP-13. For optimal detection, SW620 cells should be treated with TPA (100 ng/ml) to induce expression of MMP-7. Antibody should be titrated for optimal results in individual systems.

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

12 - Non Combustible Liquids

WGK 1