Anti-MT1-MMP (Ab-4) Mouse mAb (113-5B7)

100

mouse

purified antibody

primary antibodies

113-5B7, monoclonal

liquid

≤0.1% sodium azide as preservative

rabbit, mouse, rat, human

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG3

wet ice

−20℃

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Recognizes the ~60 kDa MTI-MMP protein in HT-1080 cells and in ConA-treated MD-MB-231 cells.
This Anti-MT1-MMP (Ab-4) Mouse mAb (113-5B7) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of MT1-MMP (Ab-4).
Purified mouse monoclonal antibody. Recognizes the ~60 kDa MTI-MMP protein in overexpressing cells.

a synthetic peptide (CDGNFDTVAMLRGEM) corresponding to amino acids 319-333 in the hemopexin-like domain of human MT1-MMP
Human

Immunoblotting (10 g/ml)
Immunofluorescence (see application references)
Immunoprecipitation (see application references)
Paraffin Sections (see application references)

Toxicity: Standard Handling (A)

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
HT-1080 or ConA-treated MD-MB-231 cells

Sakakibara, M., et al. 1999. Cancer85, 231-239.
Mattei, M.G., et al. 1997. Genomics:40, 168.
Okada, A., et al. 1997. J. Cell. Biol.147, 67.
Okumura, Y., et al. 1997. FEBS Lett.402, 181.
Ueno, H., et al. 1997. Cancer Res.57, 2055.
Strongin, A.Y., et al. 1995. J. Biol. Chem.270 5331.
Takino, T., et al. 1995. J. Biol. Chem.270, 23013.
Cottam, D. W. and Rees, R. C., 1993. Intl J. Oncol.2, 861.
Stetler-Stevenson, W. G., et al. 1993. FASEB J.7, 1434.
Fridman, R., et al. 1992. J. Biol. Chem.267, 15389.
Woessner, J. F., 1991. FASEB J.5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Goldberg, G.I., et al. 1989. PNAS86, 8207.
This antibody cross-reacts slightly with human MMP-3. To induce MT1-MMP in MD-MB-231 cells, treat with 1-5 µg/ml ConA for 24-48 h. Antibody should be titrated for optimal results in individual systems.

Manufactured by Daiichi Fine Chemicals C0., Ltd., Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

12 - Non Combustible Liquids

WGK 1