Anti-Neogenin Antibody, clone 21A8

32160702

Neogenin (UniProt: P97798) is encoded by the Neo1 (also known as Ngn) gene in murine species. Neogenin is a single-pass type I membrane protein that is expressed ubiquitously throughout the mid to late stages of gestation and in adult tissues. It strong expression is observed in the ventral region of the ventricular zone of the E15.5 mouse neural tube, as well as in the ventricular zones of the mesencephalon and rhombencephalon. Five different isoforms of neogenin have been described that are produced by alternative splicing. Isoform 3 and isoform 4 are shown to be expressed at higher level compared to other isoforms between E11.5 and E16.5. Neogenin is a multi-functional cell surface receptor that regulates cell adhesion in many diverse developmental processes, including neural tube and mammary gland formation, myogenesis and angiogenesis. It also serves as a receptor for members of the BMP, netrin, and repulsive guidance molecule (RGM) families. Interactions between netrin and neogenin result in a chemoattractive axon guidance response and cell-cell adhesion whereas its interaction with RGMa and RGMb can induce a chemorepulsive response. Neogenin is synthesized with a signal peptide (aa 1-36), which is subsequently cleaved off to produce the mature form that has an extracellular domain (aa 37-1136), a short transmembrane domain (aa 1137-1157), and a cytoplasmic domain (aa 1158-1493).

Clone 21A8 is a rat monoclonal antibody that specifically detects murine Neuogenin. It targets an epitope with in the extracellular domain.

Murine Neogenin ectodomain region fused to N-terminal CD5 signal peptide and a C-terminal 2X StrepII Tag.

Epitope: extracellular domain

Immunocytochemistry Analysis: A representative lot detected Neogenin in Immunocytochemistry applications (Kuhn, P.H., et. al. (2016). Elife. 5. pii: e12748).

Western Blotting Analysis: A representative lot detected Neogenin in Western Blotting applications (Kuhn, P.H., et. al. (2016). Elife. 5. pii: e12748).

Research Category
Neuroscience
Anti-Neogenin, clone 21A8 Antibody, Cat. No. MABN2316, is a highly specific rat monoclonal antibody that detects murine Neogenin and has been tested for use in Immunocytochemistry and Western Blotting.

Evaluated by Western Blotting in Non-reduced NIH/3T3 cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected Neogenin in Non-reduced NIH/3T3 cell lysates.

Protein G purified
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Concentration: Please refer to lot specific datasheet.

rat

purified immunoglobulin

primary antibodies

21A8, monoclonal

mouse

antibody small pack of 25 μg

immunocytochemistry: suitable
western blot: suitable

IgG1κ

P97798

~163 kDa observed; 163.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.